mouse anti ac α tub  (Santa Cruz Biotechnology)

Journal: Neural Development

Article Title: Capitella teleta gets left out: possible evolutionary shift causes loss of left tissues rather than increased neural tissue from dominant-negative BMPR1

doi: 10.1186/s13064-024-00181-7

Figure Lengend Snippet: Ct-Chd-l CRISPR phenotypes. A – E Control, uninjected animal. F – J Asymmetrical Ct-Chd-l CRISPR animals showing loss of left mesoderm (arrowhead) and reduced left foregut (arrow). Note: The apparent asymmetrical brain is an imaging artefact; F is a different animal than G – J . K – O Ct-Chd-l CRISPR animal with a wide VNC; arrowhead: separated prototroch. P Summary of asymmetrical phenotypes. A , F , K Anterior brain showing the number of SC ac+ cells. B , G , L merged images; C , H , M nuclei (cyan); D , I , N Acetylated tubulin (yellow); E , J , O Serotonin (magenta); Scale bars: 50 µm; A: anterior; D: dorsal; L, left lateral; br: brain; fg: foregut; nt: neurotroch

Article Snippet: Primary antibodies used were as follows: 1:800 rabbit anti-serotonin (5HT; Sigma-Aldrich, cat. S5545), 1:20 mouse anti-Futsch (clone 22C10, Developmental Studies Hybridoma Bank), 1:800 mouse anti-acetylated-Tubulin (ac-Tub; clone 6-11B-1, Sigma, cat. T6793), and 1:400 rabbit anti-phosphorylated-SMAD1/5/8 (pSMAD1/5/8; clone 41D10, Cell Signaling Technologies).

Techniques: CRISPR, Imaging

Journal: FASEB BioAdvances

Article Title: Ciliary GPCR‐based transcriptome as a key regulator of cilia length control

doi: 10.1096/fba.2021-00029

Figure Lengend Snippet: Search for genes involved in MCH‐MCHR1‐mediated cilia shortening by RNA‐seq analysis

Article Snippet: The cells grown on Lab‐Tek plates or coverslips were fixed with 3.7% formaldehyde in PBS for 15 min. After two washes with PBS, the cells were permeabilized with 0.1% Triton X‐100, transferred into a blocking solution (20% goat serum in PBS) for 30 min, and incubated with mouse anti‐acetylated tubulin (Ac‐tub) (T7451; Sigma‐Aldrich; 1:2000) and rabbit anti‐GFP (598; MBL; 1:2000) primary antibodies for 16–24 h at 4°C.

Techniques: Activity Assay